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Anti-Smoothelin Polyclonal Antibody
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Catalog No. TMAB-12981
Anti-Smoothelin Polyclonal Antibody is a Rabbit antibody targeting Smoothelin. Anti-Smoothelin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Smoothelin Polyclonal Antibody
Anti-Smoothelin Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-12981
Anti-Smoothelin Polyclonal Antibody is a Rabbit antibody targeting Smoothelin. Anti-Smoothelin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Smoothelin Polyclonal Antibody is a Rabbit antibody targeting Smoothelin. Anti-Smoothelin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit, Orangutan) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Smoothelin) Polyclonal Antibody, Unconjugated (TMAB-12981) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 2. Sample: Lane 1: Human MCF-7 cell lysates Lane 2: Human K562 cell lysates Lane 3: Human Jurkat cell lysates Primary: Anti-Smoothelin (TMAB-12981) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kDa Observed band size: 115 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm > cytoskeleton. Exhibits a filamentous organization. |
| Tissue Specificity | Smooth muscle; contractile or vascular (for the long form). |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The cytoskeletal protein smoothelin is highly conserved among vertebrates and is expressed exclusively by contractile smooth muscle cells where it localizes to the filament network. Smoothelin associates with actin stress fibers but does not interact with desmin. At least two isoforms of smoothelin are produced by alternative splicing. The short isoform lacks amino acids 1–544 at the amino-terminus of the long isoform. The short isoform is expressed in visceral muscle tissue, including intestine and stomach, but not in brain, while the long isoform is expressed in all vascularized organs. In the vascular system, smoothelin expression is limited to large veins and arteries capable of pulsatile contraction. As a marker for the highly differentiated contractile smooth muscle cell, smoothelin expression is useful for studying vascular malformation and injury. The gene encoding human smoothelin maps to chromosome 22q12.2. |
| Immunogen | KLH conjugated synthetic peptide: human Smoothelin |
| Antigen Species | Human |
| Gene Name | SMTN |
| Gene ID | |
| Protein Name | Smoothelin |
| Uniprot ID | |
| Function | Structural protein of the cytoskeleton. |
| Molecular Weight | Theoretical: 100 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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