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Anti-SMARCC1 Antibody (6J524) is a Rabbit antibody targeting SMARCC1. Anti-SMARCC1 Antibody (6J524) can be used in WB,ICC,IF,FCM,IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-SMARCC1 Antibody (6J524) is a Rabbit antibody targeting SMARCC1. Anti-SMARCC1 Antibody (6J524) can be used in WB,ICC,IF,FCM,IP. |
Synonyms | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, SWI/SNF complex subunit SMARCC1, SWI/SNF complex 155 kDa subunit, SMARCC1, BRG1-associated factor 155 (BAF155), BAF155 |
Ig Type | IgG |
Reactivity | Human,Rat |
Verified Activity | 1. Western blot analysis of SMARCC1 on different cell lysates using anti-SMARCC1 at 1/500 dilution. Positive control: Line 1: Mouse thymus tissue, Line 2: HL-60. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SMARCC1 antibody. Counter stained with hematoxylin. 3. ICC staining SMARCC1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of K562 cells with SMARCC1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:500-1000; ICC: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. May stimulate the ATPase activity of the catalytic subunit of the complex. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 123 (Observed: 150) kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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