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Anti-SMARCA4 Antibody (3I277)
Catalog No. TMAC-00476 Copy Product Info
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Anti-SMARCA4 Antibody (3I277) is a Rabbit antibody targeting SMARCA4. Anti-SMARCA4 Antibody (3I277) can be used in ICC/IF,IHC,IP,WB.
Anti-SMARCA4 Antibody (3I277)
Copy Product Info🥰Excellent
Catalog No. TMAC-00476
Anti-SMARCA4 Antibody (3I277) is a Rabbit antibody targeting SMARCA4. Anti-SMARCA4 Antibody (3I277) can be used in ICC/IF,IHC,IP,WB.
Anti-SMARCA4 Antibody
(3I277)
(3I277)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $297 | 7-10 days | 7-10 days | |
| 100 μL | $496 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-SMARCA4 Antibody (3I277) is a Rabbit antibody targeting SMARCA4. Anti-SMARCA4 Antibody (3I277) can be used in ICC/IF,IHC,IP,WB. |
| Ig Type | IgG |
| Clone | 3I277 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of BRG1 on different lysates using anti-BRG1 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: HepG2. 2. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-BRG1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BRG1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-BRG1 antibody. Counter stained with hematoxylin. 7. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-BRG1 antibody. Counter stained with hematoxylin. 8. ICC staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining BRG1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. ICC staining BRG1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 11. ICC staining BRG1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.            |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor). |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 185 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Keywords
Transcription activator BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4SNF2-βSNF2βSNF2L4SNF2L 4SNF2-betaSNF2-bSNF2BSMARCA4SMARCA 4Protein BRG-1Protein BRG1Protein brahma homolog 1Mitotic growth and transcription activatorBRG1-associated factor 190A (BAF190A)BRG1BRG 1BAF190ABAF 190AATP-dependent helicase SMARCA4
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