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Anti-SMAD7 Antibody (3G366)
Catalog No. TMAB-12946 Copy Product Info
🥰Excellent
Anti-SMAD7 Antibody (3G366) is a Mouse antibody targeting SMAD7. Anti-SMAD7 Antibody (3G366) can be used in WB.
Anti-SMAD7 Antibody (3G366)
Copy Product Info🥰Excellent
Catalog No. TMAB-12946
Anti-SMAD7 Antibody (3G366) is a Mouse antibody targeting SMAD7. Anti-SMAD7 Antibody (3G366) can be used in WB.
Anti-SMAD7 Antibody
(3G366)
(3G366)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-SMAD7 Antibody (3G366) is a Mouse antibody targeting SMAD7. Anti-SMAD7 Antibody (3G366) can be used in WB. |
| Ig Type | IgG |
| Clone | 3G366 |
| Reactivity | Human (predicted:Mouse,Rat) |
| Verified Activity | 1. Sample: Lane 1: Human Hela cell Lysates Lane 2: Human 293T cell Lysates Primary: Anti-SMAD7 (TMAB-12946) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SMAD7) Monoclonal Antibody, Unconjugated (TMAB-12946) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SMAD7) Monoclonal Antibody, Unconjugated (TMAB-12946) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SMAD7) Monoclonal Antibody, Unconjugated (TMAB-12946) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB=1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Nucleus. Cytoplasm. Note=Interaction with NEDD4L or RNF111 or induces translocation from the nucleus to the cytoplasm. TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta. |
| Tissue Specificity | Ubiquitous with higher expression in the lung and vascular endothelium. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Immunogen | KLH conjugated synthetic peptide: human SMAD7 |
| Antigen Species | Human |
| Gene Name | SMAD7 |
| Gene ID | |
| Protein Name | Mothers against decapentaplegic homolog 7 |
| Uniprot ID | |
| Function | Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Molecular Weight | Theoretical: 46 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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