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Anti-SMAD5 Antibody (9J928)
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Catalog No. TMAC-03799
Alias SMAD family member 5, SMAD 5, SMAD, SMA and MAD related protein 5, MusMLP, mothers against DPP homolog 5, Mothers against DPP homolog 5, mothers against decapentaplegic homolog 5, MADH5, MADH 5, MAD homolog 5, MAD, JV5 1, hSmad5, Dwfc, DKFZp781O1323, DKFZp781C1895
Anti-SMAD5 Antibody (9J928) is a Rabbit antibody targeting SMAD5. Anti-SMAD5 Antibody (9J928) can be used in WB,ICC,IHC,FCM.
Anti-SMAD5 Antibody (9J928)
Anti-SMAD5 Antibody (9J928)
😃Good
Catalog No. TMAC-03799Alias SMAD family member 5, SMAD 5, SMAD, SMA and MAD related protein 5, MusMLP, mothers against DPP homolog 5, Mothers against DPP homolog 5, mothers against decapentaplegic homolog 5, MADH5, MADH 5, MAD homolog 5, MAD, JV5 1, hSmad5, Dwfc, DKFZp781O1323, DKFZp781C1895
Anti-SMAD5 Antibody (9J928) is a Rabbit antibody targeting SMAD5. Anti-SMAD5 Antibody (9J928) can be used in WB,ICC,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-SMAD5 Antibody (9J928) is a Rabbit antibody targeting SMAD5. Anti-SMAD5 Antibody (9J928) can be used in WB,ICC,IHC,FCM. |
| Synonyms | SMAD family member 5, SMAD 5, SMAD, SMA and MAD related protein 5, MusMLP, mothers against DPP homolog 5, Mothers against DPP homolog 5, mothers against decapentaplegic homolog 5, MADH5, MADH 5, MAD homolog 5, MAD, JV5 1, hSmad5, Dwfc, DKFZp781O1323, DKFZp781C1895 |
| Ig Type | IgG |
| Reactivity | Human,Rat |
| Verified Activity | 1. Western blot analysis of Smad5 on PC-3M cell lysates using anti-Smad5 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Smad5 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Smad5 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Smad5 antibody. Counter stained with hematoxylin. 5. ICC staining Smad5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Smad5 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Smad5 in PC-3M cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with Smad5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 52 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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