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Anti-SMAD4 Antibody (1D720)
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Catalog No. TMAB-01741
Alias SMAD4, SMAD Family Member 4, SMAD 4, Mothers Against DPP Homolog 4, Mothers Against Decapentaplegic Homolog 4, MADH4, MAD Homolog 4, hSMAD4, DPC4, Deletion Target in Pancreatic Carcinoma 4
Anti-SMAD4 Antibody (1D720) is a Rabbit antibody targeting SMAD4. Anti-SMAD4 Antibody (1D720) can be used in IF,IHC-Fr,IHC-P,WB.
Anti-SMAD4 Antibody (1D720)
Anti-SMAD4 Antibody (1D720)
😃Good
Catalog No. TMAB-01741Alias SMAD4, SMAD Family Member 4, SMAD 4, Mothers Against DPP Homolog 4, Mothers Against Decapentaplegic Homolog 4, MADH4, MAD Homolog 4, hSMAD4, DPC4, Deletion Target in Pancreatic Carcinoma 4
Anti-SMAD4 Antibody (1D720) is a Rabbit antibody targeting SMAD4. Anti-SMAD4 Antibody (1D720) can be used in IF,IHC-Fr,IHC-P,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $273 | 7-10 days | 7-10 days | |
| 100 μL | $487 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-SMAD4 Antibody (1D720) is a Rabbit antibody targeting SMAD4. Anti-SMAD4 Antibody (1D720) can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | SMAD4, SMAD Family Member 4, SMAD 4, Mothers Against DPP Homolog 4, Mothers Against Decapentaplegic Homolog 4, MADH4, MAD Homolog 4, hSMAD4, DPC4, Deletion Target in Pancreatic Carcinoma 4 |
| Ig Type | IgG |
| Clone | 1D720 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Lung (Mouse) Lysate at 40 μg Primary: Anti-Smad4 (TMAB-01741) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa 2. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smad4 Monoclonal Antibody, Unconjugated (TMAB-01741) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smad4 Monoclonal Antibody, Unconjugated (TMAB-01741) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smad4 Monoclonal Antibody, Unconjugated (TMAB-01741) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smad4 Monoclonal Antibody, Unconjugated (TMAB-01741) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.      |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R-SMAD. PDPK1 prevents its nuclear translocation in response to TGF-beta. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Smad 4 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 4 is the common Smad (co-Smad). Previously identified as the tumor suppressor DPC4 (deleted in pancreatic carcinoma, locus 4), Smad 4 is functionally distinct among the Smad family, and is required for the assembly and transcriptional activation of diverse, Smad-DNA complexes. In contrast to the R-Smads, Smad 4 is not regulated by phosphorylation, but acts as a common mediator of TGF-Beta, activin, and bone morphogenetic protein signaling responses. Smad 4 is frequently inactivated in pancreatic, biliary and colorectal tumors. |
| Immunogen | A synthesized peptide: human SMAD4 |
| Antigen Species | Human |
| Gene Name | SMAD4 |
| Gene ID | |
| Protein Name | Mothers against decapentaplegic homolog 4 |
| Uniprot ID | |
| Biology Area | Response to hypoxia,Tumor suppressors,Apoptosis,Transcription factors/regulators,Transcription factors,Endothelium,SMADs,Hypoxia,SMADs,Cytoplasmic |
| Function | Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for syngernistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Molecular Weight | Theoretical: 60 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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