Anti-SMAD3 Antibody (6Y358) is a Rabbit antibody targeting SMAD3. Anti-SMAD3 Antibody (6Y358) can be used in WB,ICC/IF,IHC,FCM.

IgG

Human,Mouse,Rat

1. Western blot analysis of Smad3 on A549 cell lysates using anti-Smad3 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Smad3 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Smad3 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Smad3 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Smad3 antibody. Counter stained with hematoxylin.
6. ICC staining Smad3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Smad3 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining Smad3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. Flow cytometric analysis of Hela cells with Smad3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100

Monoclonal

Unconjugated

Recombinant Protein