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Anti-SMAD2 Antibody (4E423)
😃Good
Catalog No. TMAC-03786
Alias SMAD2, MADR2, MADH2, LDS6, JV18-1, JV18, hSMAD2, hMAD-2, CHTD8
Anti-SMAD2 Antibody (4E423) is a Rabbit antibody targeting SMAD2. Anti-SMAD2 Antibody (4E423) can be used in WB,ICC,IHC,IP,FCM.
Anti-SMAD2 Antibody (4E423)
Anti-SMAD2 Antibody (4E423)
😃Good
Catalog No. TMAC-03786Alias SMAD2, MADR2, MADH2, LDS6, JV18-1, JV18, hSMAD2, hMAD-2, CHTD8
Anti-SMAD2 Antibody (4E423) is a Rabbit antibody targeting SMAD2. Anti-SMAD2 Antibody (4E423) can be used in WB,ICC,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-SMAD2 Antibody (4E423) is a Rabbit antibody targeting SMAD2. Anti-SMAD2 Antibody (4E423) can be used in WB,ICC,IHC,IP,FCM. |
| Synonyms | SMAD2, MADR2, MADH2, LDS6, JV18-1, JV18, hSMAD2, hMAD-2, CHTD8 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Smad2 on different lysates using anti-Smad2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: A431, Lane 3: PC12. 2. Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-Smad2 antibody. Counter stained with hematoxylin. 3. ICC staining Smad2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Smad2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Smad2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of NIH/3T3 cells with Smad2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 58 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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