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Anti-SMAD1/SMAD5 Polyclonal Antibody is a Rabbit antibody targeting SMAD1/SMAD5. Anti-SMAD1/SMAD5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-SMAD1/SMAD5 Polyclonal Antibody is a Rabbit antibody targeting SMAD1/SMAD5. Anti-SMAD1/SMAD5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rabbit (predicted:Rat,Chicken,Dog,Cow,Horse) |
Verified Activity | 1. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (SMAD1 + SMAD5) polyclonal Antibody, Unconjugated (TMAB-01735) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SMAD1 + SMAD5) Polyclonal Antibody, Unconjugated (TMAB-01735) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Primary: Anti-SMAD1 + SMAD5 (TMAB-01735) at 1/200 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 55 kDa |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
Recommended Dose | WB: 1:200-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | KLH conjugated synthetic peptide: human SMAD5 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Liver development,SMADs,SMADs,Cytoplasmic |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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