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Anti-SMAD1/SMAD5 Polyclonal Antibody

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Catalog No. TMAB-01735

Anti-SMAD1/SMAD5 Polyclonal Antibody is a Rabbit antibody targeting SMAD1/SMAD5. Anti-SMAD1/SMAD5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-SMAD1/SMAD5 Polyclonal Antibody

Anti-SMAD1/SMAD5 Polyclonal Antibody

😃Good
Catalog No. TMAB-01735
Anti-SMAD1/SMAD5 Polyclonal Antibody is a Rabbit antibody targeting SMAD1/SMAD5. Anti-SMAD1/SMAD5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$374 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-SMAD1/SMAD5 Polyclonal Antibody is a Rabbit antibody targeting SMAD1/SMAD5. Anti-SMAD1/SMAD5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rabbit (predicted:Rat,Chicken,Dog,Cow,Horse)
Verified Activity
1. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (SMAD1 + SMAD5) polyclonal Antibody, Unconjugated (TMAB-01735) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SMAD1 + SMAD5) Polyclonal Antibody, Unconjugated (TMAB-01735) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-SMAD1 + SMAD5 (TMAB-01735) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 55 kDa
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:200-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human SMAD5
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Liver development,SMADs,SMADs,Cytoplasmic
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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