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Anti-SMAD1/SMAD5 Polyclonal Antibody
😃Good
Catalog No. TMAB-01735
Alias SMAD5, SMAD mothers against DPP homolog 5, SMAD family member 5, SMAD 5, SMA and MAD related protein 5, MusMLP, Mothers against DPP homolog 5, Mothers against decapentaplegic homolog 5, MADH5, MADH 5, MAD, mothers against decapentaplegic homolog 5, MAD mothers against decapentaplegic homolog 5, MAD homolog 5, JV5-1, hSmad5, hSmad 5, Dwfc
Anti-SMAD1/SMAD5 Polyclonal Antibody is a Rabbit antibody targeting SMAD1/SMAD5. Anti-SMAD1/SMAD5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-SMAD1/SMAD5 Polyclonal Antibody
Anti-SMAD1/SMAD5 Polyclonal Antibody
😃Good
Catalog No. TMAB-01735Alias SMAD5, SMAD mothers against DPP homolog 5, SMAD family member 5, SMAD 5, SMA and MAD related protein 5, MusMLP, Mothers against DPP homolog 5, Mothers against decapentaplegic homolog 5, MADH5, MADH 5, MAD, mothers against decapentaplegic homolog 5, MAD mothers against decapentaplegic homolog 5, MAD homolog 5, JV5-1, hSmad5, hSmad 5, Dwfc
Anti-SMAD1/SMAD5 Polyclonal Antibody is a Rabbit antibody targeting SMAD1/SMAD5. Anti-SMAD1/SMAD5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-SMAD1/SMAD5 Polyclonal Antibody is a Rabbit antibody targeting SMAD1/SMAD5. Anti-SMAD1/SMAD5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Alias | SMAD5, SMAD mothers against DPP homolog 5, SMAD family member 5, SMAD 5, SMA and MAD related protein 5, MusMLP, Mothers against DPP homolog 5, Mothers against decapentaplegic homolog 5, MADH5, MADH 5, MAD, mothers against decapentaplegic homolog 5, MAD mothers against decapentaplegic homolog 5, MAD homolog 5, JV5-1, hSmad5, hSmad 5, Dwfc |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rabbit (predicted:Rat,Chicken,Dog,Cow,Horse) |
| Verified Activity | 1. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (SMAD1 + SMAD5) polyclonal Antibody, Unconjugated (TMAB-01735) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SMAD1 + SMAD5) Polyclonal Antibody, Unconjugated (TMAB-01735) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Primary: Anti-SMAD1 + SMAD5 (TMAB-01735) at 1/200 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 55 kDa |
| Application | |
| Recommended Dose | WB: 1:200-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide: human SMAD5 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Liver development,SMADs,SMADs,Cytoplasmic |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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