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Anti-SMAD1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01734
Anti-SMAD1 Polyclonal Antibody is a Rabbit antibody targeting SMAD1. Anti-SMAD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-SMAD1 Polyclonal Antibody
Anti-SMAD1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01734
Anti-SMAD1 Polyclonal Antibody is a Rabbit antibody targeting SMAD1. Anti-SMAD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-SMAD1 Polyclonal Antibody is a Rabbit antibody targeting SMAD1. Anti-SMAD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SMAD1) Polyclonal Antibody, Unconjugated (TMAB-01734) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Tissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-MDFI Polyclonal Antibody, Unconjugated (TMAB-01734) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: MCF-7 (Human) Cell Lysate at 30 μg Primary: Anti-SMAD1 (TMAB-01734) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa 4. Sample: H9C2 (Rat) Cell Lysate at 30 μg Primary: Anti-SMAD1 (TMAB-01734) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa 5. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SMAD1) Polyclonal Antibody, Unconjugated (TMAB-01734) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Sample: Heart (Mouse) Lysate at 40 μg Muscle (Mouse) Lysate at 40 μg Primary: Anti-SMAD1 (TMAB-01734) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Research Background | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human SMAD1 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | TGF,SMADs,SMADs,Cytoplasmic |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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