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Anti-SMAC Antibody (6G724)
😃Good
Catalog No. TMAY-00649
Alias SMAC, diablo, IAP-binding mitochondrial protein, DFNA64
Anti-SMAC Antibody (6G724) is a Mouse antibody targeting SMAC. Anti-SMAC Antibody (6G724) can be used in ICC/IF.
Anti-SMAC Antibody (6G724)
Anti-SMAC Antibody (6G724)
😃Good
Catalog No. TMAY-00649Alias SMAC, diablo, IAP-binding mitochondrial protein, DFNA64
Anti-SMAC Antibody (6G724) is a Mouse antibody targeting SMAC. Anti-SMAC Antibody (6G724) can be used in ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-SMAC Antibody (6G724) is a Mouse antibody targeting SMAC. Anti-SMAC Antibody (6G724) can be used in ICC/IF. |
| Alias | SMAC, diablo, IAP-binding mitochondrial protein, DFNA64 |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 6G724 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of Human Diablo in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human Diablo monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue). Positive staining was localized to cytoplasm. |
| Application | ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SMAC / Diablo (rh SMAC / Diablo ; TMPY-00924; NP_063940.1; Ala56-Asp239). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human SMAC / Diablo protein (TMPY-00924) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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