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Anti-SMAC Antibody (5G346)
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Catalog No. TMAY-00650
Alias SMAC, diablo, IAP-binding mitochondrial protein, DFNA64
Anti-SMAC Antibody (5G346) is a Rabbit antibody targeting SMAC. Anti-SMAC Antibody (5G346) can be used in IP.
Anti-SMAC Antibody (5G346)
Anti-SMAC Antibody (5G346)
😃Good
Catalog No. TMAY-00650Alias SMAC, diablo, IAP-binding mitochondrial protein, DFNA64
Anti-SMAC Antibody (5G346) is a Rabbit antibody targeting SMAC. Anti-SMAC Antibody (5G346) can be used in IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-SMAC Antibody (5G346) is a Rabbit antibody targeting SMAC. Anti-SMAC Antibody (5G346) can be used in IP. |
| Alias | SMAC, diablo, IAP-binding mitochondrial protein, DFNA64 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 5G346 |
| Reactivity | Human |
| Verified Activity | Diablo was immunoprecipitated using: -Lane A:0.5 mg 293T Whole Cell Lysate. -Lane B:0.5 mg MCF-7 Whole Cell Lysate. -Lane C:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-Diablo rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Diablo rabbit monoclonal antibody, at 1:330 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 19 kDa. -Observed band size: 19 kDa |
| Application | IP |
| Recommended Dose | IP: 0.5-2 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human SMAC / Diablo (rh SMAC / Diablo; TMPY-00924; NP_063940.1; Ala56-Asp239). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human SMAC / Diablo Protein (TMPY-00924) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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