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Anti-SLC5A5 Polyclonal Antibody is a Rabbit antibody targeting SLC5A5. Anti-SLC5A5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-SLC5A5 Polyclonal Antibody is a Rabbit antibody targeting SLC5A5. Anti-SLC5A5 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | Solute carrier family 5 member 5, Sodium-iodide symporter (Na(+)/I(-) symporter), Sodium/iodide cotransporter, SLC5A5, NIS, Natrium iodide transporter, Na(+)/I(-) cotransporter |
Ig Type | IgG |
Reactivity | Mouse,Rat (predicted:Human) |
Verified Activity | 1. Tissue/cell: rat thyroid gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-NIS Polyclonal Antibody, Unconjugated (TMAB-01727) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-NIS Polyclonal Antibody, Unconjugated (TMAB-01727) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Lane 1: Mouse Stomach tissue lysates Lane 2: Rat Stomach tissue lysates Primary: Anti-NIS (TMAB-01727) at 1/200 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kDa Observed band size: 48 kDa |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Membrane; Multi-pass membrane protein. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | catalyzes Na+/I- symporter activity plays a role in iodide transport and thyroid hormone generation. Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane bound glycoprotein with 13 membrane spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid. |
Immunogen | KLH conjugated synthetic peptide: human NIS |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Vitamins / Minerals,Channels,Hormone biosynthesis,Vitamins / minerals,Hormone biosynthesis |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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