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Anti-SLC2A6 Polyclonal Antibody

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Catalog No. TMAB-01717

Anti-SLC2A6 Polyclonal Antibody is a Rabbit antibody targeting SLC2A6. Anti-SLC2A6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-SLC2A6 Polyclonal Antibody

Anti-SLC2A6 Polyclonal Antibody

😃Good
Catalog No. TMAB-01717
Anti-SLC2A6 Polyclonal Antibody is a Rabbit antibody targeting SLC2A6. Anti-SLC2A6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$374 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-SLC2A6 Polyclonal Antibody is a Rabbit antibody targeting SLC2A6. Anti-SLC2A6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human)
Verified Activity
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GLUT6 Polyclonal Antibody, Unconjugated (TMAB-01717) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GLUT6 Polyclonal Antibody, Unconjugated (TMAB-01717) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Brain (Mouse) Lysate at 40 μg
Primary: Anti-GLUT6/GLUT9 (TMAB-01717) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
4. Sample:
Heart (Mouse) Lysate at 40 μg
Primary: Anti-GLUT6/GLUT9 (TMAB-01717) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane. The dileucine internalization motif is critical for intracellular sequestration.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe oxidation of glucose functions as the dominant source of metabolic energy for mammals. The plasma membrane is impermeable to glucose, so the cellular uptake of this important nutrient is achieved by facultative hexose transproters (Gluts). Gluts are integral membrane proteins that transport glucose and related hexoses. Glucose binds to a Glut on one side of the membrane which provokes a conformational change causing it to release glucose to the other side. Members of the Glut family may enhance the metabolic activity of tumor cells. Glut6 is part of the third out of three classes of Gluts. Glut6 is mainly expressed in the brain, spleen and peripheral leukocytes. It appears to be regulated by subcellular redistribution, because it is targeted to intracellular compartments by di-leucine motifs, recycling itself in a Dynamin-dependent manner.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GLUT6
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cancer,Channels
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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