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Anti-SLC2A4 Polyclonal Antibody is a Rabbit antibody targeting SLC2A4. Anti-SLC2A4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-SLC2A4 Polyclonal Antibody is a Rabbit antibody targeting SLC2A4. Anti-SLC2A4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit,Sheep) |
Verified Activity | 1. Sample: Heart (Rat) Cell Lysate at 40 μg Primary: Anti-GLUT4 (TMAB-01715) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa 2. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated (TMAB-01715) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated (TMAB-01715) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Blank control (blue line): K562 (blue). Primary Antibody (green line): Rabbit Anti-GLUT4 antibody (TMAB-01715) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1 μg/test. Protocol The cells were fixed with 70% ethanol (overnight at 4°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. 5. Sample: Heart (Mouse) Lysate at 40 μg Primary: Anti-GLUT4 (TMAB-01715) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa 6. Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 2: Heart (Mouse) Lysate at 40 μg Lane 3: Heart (Rat) Lysate at 40 μg Lane 4: Muscle (Mouse) Lysate at 40 μg Lane 5: Muscle (Rat) Lysate at 40 μg Primary: Anti-GLUT4 (TMAB-01715) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa 7. Tissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (GLUT4) polyclonal Antibody, Unconjugated (TMAB-01715) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1μg/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Endomembrane system. Cytoplasm > perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | GLUT4 is the facilitated glucose transporter expressed exclusively in adipocytes and muscle cells, and is also known as the "insulin-responsive" glucose transporter. GLUT4 translocates from an ill-defined intracellular compartment to the plasma membrane in response to insulin. The total cellular content of GLUT4 is significantly decreased in adipose cells from many patients with Type II diabetes mellitus, and animals with some types of experimental diabetes. |
Immunogen | KLH conjugated synthetic peptide: human GLUT4 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Metabolism of carbohydrates,Cardiac metabolism,Carbohydrate metabolism,Energy Metabolism,Diabetes,Energy Metabolism,Adipogenesis |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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