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Anti-SLC2A3 Polyclonal Antibody

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Catalog No. TMAB-01714
Alias Solute Carrier Family 2(Facilitated Glucose Transporter) Member 3, Solute carrier family 2, GTR3, GLUT 3, Glucose transporter type 3 brain, Glucose Transporter Type 3, Glucose Transporter GLUT3, FLJ90380, facilitated glucose transporter member 3

Anti-SLC2A3 Polyclonal Antibody is a Rabbit antibody targeting SLC2A3. Anti-SLC2A3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-SLC2A3 Polyclonal Antibody

Anti-SLC2A3 Polyclonal Antibody

😃Good
Catalog No. TMAB-01714Alias Solute Carrier Family 2(Facilitated Glucose Transporter) Member 3, Solute carrier family 2, GTR3, GLUT 3, Glucose transporter type 3 brain, Glucose Transporter Type 3, Glucose Transporter GLUT3, FLJ90380, facilitated glucose transporter member 3
Anti-SLC2A3 Polyclonal Antibody is a Rabbit antibody targeting SLC2A3. Anti-SLC2A3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3737-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-SLC2A3 Polyclonal Antibody is a Rabbit antibody targeting SLC2A3. Anti-SLC2A3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasSolute Carrier Family 2(Facilitated Glucose Transporter) Member 3, Solute carrier family 2, GTR3, GLUT 3, Glucose transporter type 3 brain, Glucose Transporter Type 3, Glucose Transporter GLUT3, FLJ90380, facilitated glucose transporter member 3
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig)
Verified Activity
1. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Glut3 Polyclonal Antibody, Unconjugated (TMAB-01714) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.
2. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Glut3 Polyclonal Antibody, FITC conjugated (TMAB-01714-FITC) 1:100, 60 minutes at 37°C;
Excitation wavelength: 488nm; Emission wavelength:519nm
3. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Glut3 Polyclonal Antibody, Unconjugated (TMAB-01714) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Sample:
293t (Human) Cell Lysate at 30 μg
Hela (Human) Cell Lysate at 30 μg
Primary: Anti-TMAB-01714 (TMAB-01714) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 63 kDa
5. Sample:
Brain (Mouse) Lysate at 40 μg
Brain (Rat) Lysate at 40 μg
293T (Human) Cell Lysate at 30 μg
NIH/3T3 (Mouse) CellLysate at 30 μg
Primary: Anti-GLUT3 (TMAB-01714) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50/63 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein. Melanosome.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundMay act as a glucose transporter in neurons; may mediate increasd glucose uptake in response to neuronal injury. Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF1 induced signaling. GLUT3 is detectable in a few normal cell type spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. GLUT3 staining is also detectable in human cancers including those of the ovary, lung, and testis. Alternative names: FLJ90380; Glucose Transporter Type 3; Glucose transporter type 3 brain; GLUT 3; GLUT3; SLC2A3; Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GLUT3
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Metabolism of carbohydrates,Other Antibodies,Carbohydrate metabolism,Cancer,Channels
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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