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Anti-SLC2A3 Polyclonal Antibody is a Rabbit antibody targeting SLC2A3. Anti-SLC2A3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-SLC2A3 Polyclonal Antibody is a Rabbit antibody targeting SLC2A3. Anti-SLC2A3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | Solute Carrier Family 2(Facilitated Glucose Transporter) Member 3, Solute carrier family 2, GTR3, GLUT 3, Glucose transporter type 3 brain, Glucose Transporter Type 3, Glucose Transporter GLUT3, FLJ90380, facilitated glucose transporter member 3 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig) |
Verified Activity | 1. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Glut3 Polyclonal Antibody, Unconjugated (TMAB-01714) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. 2. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Glut3 Polyclonal Antibody, FITC conjugated (TMAB-01714-FITC) 1:100, 60 minutes at 37°C; Excitation wavelength: 488nm; Emission wavelength:519nm 3. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Glut3 Polyclonal Antibody, Unconjugated (TMAB-01714) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Sample: 293t (Human) Cell Lysate at 30 μg Hela (Human) Cell Lysate at 30 μg Primary: Anti-TMAB-01714 (TMAB-01714) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 63 kDa 5. Sample: Brain (Mouse) Lysate at 40 μg Brain (Rat) Lysate at 40 μg 293T (Human) Cell Lysate at 30 μg NIH/3T3 (Mouse) CellLysate at 30 μg Primary: Anti-GLUT3 (TMAB-01714) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 50/63 kDa |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane; Multi-pass membrane protein. Melanosome. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | May act as a glucose transporter in neurons; may mediate increasd glucose uptake in response to neuronal injury. Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF1 induced signaling. GLUT3 is detectable in a few normal cell type spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. GLUT3 staining is also detectable in human cancers including those of the ovary, lung, and testis. Alternative names: FLJ90380; Glucose Transporter Type 3; Glucose transporter type 3 brain; GLUT 3; GLUT3; SLC2A3; Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3. |
Immunogen | KLH conjugated synthetic peptide: human GLUT3 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Metabolism of carbohydrates,Other Antibodies,Carbohydrate metabolism,Cancer,Channels |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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