Anti-SLC2A2 Polyclonal Antibody 2 is a Rabbit antibody targeting SLC2A2. Anti-SLC2A2 Polyclonal Antibody 2 can be used in FCM,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Horse)

1. Sample:
Lane 1: Liver (Mouse) Lysate at 40 μg
Lane 2: Kidney (Mouse) Lysate at 40 μg
Lane 3: Pancreas (Mouse) Lysate at 40 μg
Primary: Anti-GLUT2 (TMAB-01713) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57/45 kDa
Observed band size: 47 kDa
2. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GLUT2 Polyclonal Antibody, Unconjugated (TMAB-01713) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Blank control (blue line): Hep G2 (fixed with 70% ethanol Overnight at 4°C).
Primary Antibody (green line): Rabbit Anti-GLUT2 antibody (TMAB-01713), Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test.
4. Sample:
Kidney (Mouse) Lysate at 40 μg
Primary: Anti-GLUT2 (TMAB-01713) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
5. Sample:
Liver (Mouse) Lysate at 40 μg
Primary: Anti-GLUT2 (TMAB-01713) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human GLUT2

Human

SLC2A2

Solute carrier family 2, facilitated glucose transporter member 2

Metabolism of carbohydrates,Metabolism of lipids and lipoproteins,Endoderm,Carbohydrate metabolism,Energy Metabolism,Lipid metabolism,Energy Metabolism,Endoderm

Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.