Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF,FCM

Reactivity: Human,Mouse,Rat (predicted:Horse)

IgG

Human,Mouse,Rat (predicted:Horse)

1. Sample:
Lane 1: Liver (Mouse) Lysate at 40 μg
Lane 2: Kidney (Mouse) Lysate at 40 μg
Lane 3: Pancreas (Mouse) Lysate at 40 μg
Primary: Anti-GLUT2 (TMAB-01713) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57/45 kDa
Observed band size: 47 kDa
2. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GLUT2 Polyclonal Antibody, Unconjugated (TMAB-01713) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Blank control (blue line): Hep G2 (fixed with 70% ethanol Overnight at 4°C).
Primary Antibody (green line): Rabbit Anti-GLUT2 antibody (TMAB-01713), Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test.
4. Sample:
Kidney (Mouse) Lysate at 40 μg
Primary: Anti-GLUT2 (TMAB-01713) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
5. Sample:
Liver (Mouse) Lysate at 40 μg
Primary: Anti-GLUT2 (TMAB-01713) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human GLUT2

Human

6514

Metabolism of carbohydrates,Metabolism of lipids and lipoproteins,Endoderm,Carbohydrate metabolism,Energy Metabolism,Lipid metabolism,Energy Metabolism,Endoderm