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Anti-SLC2A2 Polyclonal Antibody

😃Good
Catalog No. TMAB-01712

Anti-SLC2A2 Polyclonal Antibody is a Rabbit antibody targeting SLC2A2. Anti-SLC2A2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-SLC2A2 Polyclonal Antibody

Anti-SLC2A2 Polyclonal Antibody

😃Good
Catalog No. TMAB-01712
Anti-SLC2A2 Polyclonal Antibody is a Rabbit antibody targeting SLC2A2. Anti-SLC2A2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$374 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-SLC2A2 Polyclonal Antibody is a Rabbit antibody targeting SLC2A2. Anti-SLC2A2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse,Rat,Goat (predicted:Chicken,Dog,Pig,Cow,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Glut2) Polyclonal Antibody, Unconjugated (TMAB-01712) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Blank control (blue line): Hep G2 (blue).
Primary Antibody (green line): Rabbit Anti-GLUT2 antibody (TMAB-01712)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 70% ethanol Overnight at 4°C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Pancreas (Mouse) Lysate at 40 μg
Primary: Anti-GLUT2 (TMAB-01712) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-53 kDa
Observed band size: 50 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg /test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes an integral plasma membrane glycoprotein of the liver, islet beta cells, intestine, and kidney epithelium. The encoded protein mediates facilitated bidirectional glucose transport. Because of its low affinity for glucose, it has been suggested as a glucose sensor. Mutations in this gene are associated with susceptibility to diseases, including Fanconi-Bickel syndrome and noninsulin-dependent diabetes mellitus (NIDDM). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GLUT2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Metabolism of carbohydrates,Metabolism of lipids and lipoproteins,Endoderm,Carbohydrate metabolism,Energy Metabolism,Lipid metabolism,Energy Metabolism,Endoderm
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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