Anti-SLC2A2 Polyclonal Antibody is a Rabbit antibody targeting SLC2A2. Anti-SLC2A2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat,Goat (predicted:Chicken,Dog,Pig,Cow,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Glut2) Polyclonal Antibody, Unconjugated (TMAB-01712) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Blank control (blue line): Hep G2 (blue).
Primary Antibody (green line): Rabbit Anti-GLUT2 antibody (TMAB-01712)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 70% ethanol Overnight at 4°C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Pancreas (Mouse) Lysate at 40 μg
Primary: Anti-GLUT2 (TMAB-01712) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-53 kDa
Observed band size: 50 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg /test

Polyclonal

KLH conjugated synthetic peptide: human GLUT2

Human

Metabolism of carbohydrates,Metabolism of lipids and lipoproteins,Endoderm,Carbohydrate metabolism,Energy Metabolism,Lipid metabolism,Energy Metabolism,Endoderm