Anti-SLC2A1 Polyclonal Antibody is a Rabbit antibody targeting SLC2A1. Anti-SLC2A1 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Sheep)

1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GLUT1 Polyclonal Antibody, Unconjugated (TMAB-01709) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Image was kindly submitted by Dr. Lelisa Gemta from University of Virginia. Mouse splenocytes stained with Rabbit Anti-GLUT1 Polyclonal Antibody, PE conjugated (TMAB-01709-PE) at 1:50.
3. Sample:
Lane 1: Heart (Mouse) Lysate at 40 μg
Lane 2: Cerebrum (Mouse) Lysate at 40 μg
Lane 3: Muscle (Mouse) Lysate at 40 μg
Lane 4: Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-GLUT1 (TMAB-01709) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 52 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human GLUT1

Human

SLC2A1

Solute carrier family 2, facilitated glucose transporter member 1

Metabolism of carbohydrates,Diabetes associated,Carbohydrate metabolism,Cancer,Diabetes,Heart disease,glucose transporter glut1,Channels

Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.