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Anti-SLC22A17 Polyclonal Antibody 2

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Catalog No. TMAB-01707

Anti-SLC22A17 Polyclonal Antibody 2 is a Rabbit antibody targeting SLC22A17. Anti-SLC22A17 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

Anti-SLC22A17 Polyclonal Antibody 2

Anti-SLC22A17 Polyclonal Antibody 2

😃Good
Catalog No. TMAB-01707
Anti-SLC22A17 Polyclonal Antibody 2 is a Rabbit antibody targeting SLC22A17. Anti-SLC22A17 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$373 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Sample:
Lane 1: SH-SY5Y (Human) Cell Lysate at 30 μg
Lane 2: U2os (Human) Cell Lysate at 30 μg
Lane 3: U87MG (Human) Cell Lysate at 30 μg
Lane 4: A549 (Human) Cell Lysate at 30 μg
Lane 5: Siha (Human) Cell Lysate at 30 μg
Primary:
Anti-SLC22A17 (TMAB-01707) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (TMAB-01707) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (TMAB-01707) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (TMAB-01707) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein. Note=Upon LCN2-binding, it is internalized.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundSLC22A17 (solute carrier family 22 nember 17; organic cation transportor,).may act as a brain-specific organic ion transporter. The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human SLC22A17
Antigen Species
Human
Gene ID
51310
Uniprot ID
Biology Area
Channels
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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