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Anti-SLC22A17 Polyclonal Antibody 2 is a Rabbit antibody targeting SLC22A17. Anti-SLC22A17 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep) |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep) |
Verified Activity | 1. Sample: Lane 1: SH-SY5Y (Human) Cell Lysate at 30 μg Lane 2: U2os (Human) Cell Lysate at 30 μg Lane 3: U87MG (Human) Cell Lysate at 30 μg Lane 4: A549 (Human) Cell Lysate at 30 μg Lane 5: Siha (Human) Cell Lysate at 30 μg Primary: Anti-SLC22A17 (TMAB-01707) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa 2. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (TMAB-01707) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (TMAB-01707) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (TMAB-01707) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein. Note=Upon LCN2-binding, it is internalized. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | SLC22A17 (solute carrier family 22 nember 17; organic cation transportor,).may act as a brain-specific organic ion transporter. The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. |
Immunogen | KLH conjugated synthetic peptide: human SLC22A17 |
Antigen Species | Human |
Gene ID | 51310 |
Uniprot ID | |
Biology Area | Channels |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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