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Anti-SLC1A3 Polyclonal Antibody

😃Good
Catalog No. TMAB-01705

Anti-SLC1A3 Polyclonal Antibody is a Rabbit antibody targeting SLC1A3. Anti-SLC1A3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-SLC1A3 Polyclonal Antibody

Anti-SLC1A3 Polyclonal Antibody

😃Good
Catalog No. TMAB-01705
Anti-SLC1A3 Polyclonal Antibody is a Rabbit antibody targeting SLC1A3. Anti-SLC1A3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$372 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-SLC1A3 Polyclonal Antibody is a Rabbit antibody targeting SLC1A3. Anti-SLC1A3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EAAT1) Polyclonal Antibody, Unconjugated (TMAB-01705) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EAAT1) Polyclonal Antibody, Unconjugated (TMAB-01705) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Lane 1: U87MG (Human) Cell Lysate at 30 μg
Lane 2: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-EAAT1 (TMAB-01705) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
4. Blank control: U87MG.
Primary Antibody (green line): Rabbit Anti-EAAT1 antibody (TMAB-01705)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Multi-pass membrane protein
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human EAAT1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Aspartate,Glutamate,Glutamate
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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