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Anti-SLC16A1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01702
Alias Solute carrier family 16 member 1, Monocarboxylate transporter 1, MCT1, MCT 1

Anti-SLC16A1 Polyclonal Antibody is a Rabbit antibody targeting SLC16A1. Anti-SLC16A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-SLC16A1 Polyclonal Antibody

Anti-SLC16A1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01702Alias Solute carrier family 16 member 1, Monocarboxylate transporter 1, MCT1, MCT 1
Anti-SLC16A1 Polyclonal Antibody is a Rabbit antibody targeting SLC16A1. Anti-SLC16A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-SLC16A1 Polyclonal Antibody is a Rabbit antibody targeting SLC16A1. Anti-SLC16A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
AliasSolute carrier family 16 member 1, Monocarboxylate transporter 1, MCT1, MCT 1
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,GuineaPig)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MCT1) Polyclonal Antibody, Unconjugated (TMAB-01702) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Blank control: MCF7 Cells (blue). Primary Antibody: Rabbit Anti-MCT1/AF647 Conjugated antibody (TMAB-01702-AF647), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC (orange),used under the same conditions.
3. Sample: HepG2 (Human) cell Lysate at 40 μg
Primary: Anti-MCT1 (TMAB-01702) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
4. Sample:
Lane 1: Liver (Mouse) Lysate at 40 μg
Lane 2: Stomach (Mouse) Lysate at 40 μg
Lane 3: Heart (Mouse) Lysate at 40 μg
Lane 4: Liver (Rat) Lysate at 40 μg
Lane 5: Stomach (Rat) Lysate at 40 μg
Lane 6: Heart (Rat) Lysate at 40 μg
Primary: Anti-MCT1 (TMAB-01702) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
5. Blank control: K562. Primary Antibody (green line): Rabbit Anti-MCT1 antibody (TMAB-01702)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Multi-pass membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2009]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MCT1
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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