Anti-SLC16A1 Polyclonal Antibody is a Rabbit antibody targeting SLC16A1. Anti-SLC16A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,GuineaPig)

1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MCT1) Polyclonal Antibody, Unconjugated (TMAB-01702) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Blank control: MCF7 Cells (blue). Primary Antibody: Rabbit Anti-MCT1/AF647 Conjugated antibody (TMAB-01702-AF647), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC (orange),used under the same conditions.
3. Sample: HepG2 (Human) cell Lysate at 40 μg
Primary: Anti-MCT1 (TMAB-01702) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
4. Sample:
Lane 1: Liver (Mouse) Lysate at 40 μg
Lane 2: Stomach (Mouse) Lysate at 40 μg
Lane 3: Heart (Mouse) Lysate at 40 μg
Lane 4: Liver (Rat) Lysate at 40 μg
Lane 5: Stomach (Rat) Lysate at 40 μg
Lane 6: Heart (Rat) Lysate at 40 μg
Primary: Anti-MCT1 (TMAB-01702) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
5. Blank control: K562. Primary Antibody (green line): Rabbit Anti-MCT1 antibody (TMAB-01702)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human MCT1

Human