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Anti-SKA3 Polyclonal Antibody
😃Good
Catalog No. TMAB-01696
Alias Spindle and kinetochore-associated protein 3, Spindle and kinetochore associated complex subunit 3, SKA 3, RAMA1, C13orf3
Anti-SKA3 Polyclonal Antibody is a Rabbit antibody targeting SKA3. Anti-SKA3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-SKA3 Polyclonal Antibody
Anti-SKA3 Polyclonal Antibody
😃Good
Catalog No. TMAB-01696Alias Spindle and kinetochore-associated protein 3, Spindle and kinetochore associated complex subunit 3, SKA 3, RAMA1, C13orf3
Anti-SKA3 Polyclonal Antibody is a Rabbit antibody targeting SKA3. Anti-SKA3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-SKA3 Polyclonal Antibody is a Rabbit antibody targeting SKA3. Anti-SKA3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Alias | Spindle and kinetochore-associated protein 3, Spindle and kinetochore associated complex subunit 3, SKA 3, RAMA1, C13orf3 |
| Ig Type | IgG |
| Reactivity | Rat (predicted:Human,Mouse,Chicken,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SKA3) Polyclonal Antibody, Unconjugated (TMAB-01696) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton, spindle.Chromosome, centromere, kinetochore. Note=Localizes to the outerkinetochore and spindle microtubules during mitosis in a NDC80complex-dependent manner. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. |
| Immunogen | KLH conjugated synthetic peptide: human SKA3 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Spindle |
| Molecular Weight | Theoretical: 46 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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