Anti-SIRT7 Polyclonal Antibody is a Rabbit antibody targeting SIRT7. Anti-SIRT7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Mouse (predicted:Human,Rat,Chicken,Dog,Pig)

1. Sample: Lymph node (Mouse) Lysate at 40 μg
Primary: Anti-SIRT7 (TMAB-12820) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
2. Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-SIRT7 Polyclonal Antibody, Unconjugated (TMAB-12820) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Sample: small intestine (Mouse) Lysate at 40 μg
Primary: Anti-SIRT7 (TMAB-12820) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
4. Blank control: mouse splenocytes (blue)
Isotype Control Antibody: Rabbit IgG (orange);
Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 1 μl in 100 μL1X PBS containing 0.5% BSA (green).

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human SIRT7

Human

SIRT7

RNASET2

NAD-dependent protein deacetylase. Required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis. Promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex.