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Anti-SIRT5 Polyclonal Antibody

Catalog No. TMAB-12818 Copy Product Info
🥰Excellent
Anti-SIRT5 Polyclonal Antibody is a Rabbit antibody targeting SIRT5. Anti-SIRT5 Polyclonal Antibody can be used in WB,FCM.

Anti-SIRT5 Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-12818

Anti-SIRT5 Polyclonal Antibody is a Rabbit antibody targeting SIRT5. Anti-SIRT5 Polyclonal Antibody can be used in WB,FCM.

Anti-SIRT5 Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2237-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5287-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-SIRT5 Polyclonal Antibody is a Rabbit antibody targeting SIRT5. Anti-SIRT5 Polyclonal Antibody can be used in WB,FCM.
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat)
Verified Activity
1. Blank control: mouse splenocytes (blue)
Isotype Control Antibody: Rabbit IgG (orange);
Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 1 μl in 100 μL1X PBS containing 0.5% BSA (green).
2. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-SIRT5 (TMAB-12818) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 33 kD
Application
WB
FCM
Recommended Dose
WB: 1:500-2000; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMitochondrion matrix. Mitochondrion intermembrane space.
Tissue SpecificityWidely expressed.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundSirtuins (SIRT1-7) are human homologs of the yeast Sir2 (silent information regulator-2) protein and are divided into four main classes: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. In S. cerevisiae, Sir2 deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA (ribosomal RNA) and silent mating-type loci. The human SIRT proteins are NAD-dependent deacetylases that act as intracellular regulators and are thought to have ribosyltransferase activity. SIRT5 (NAD-dependent deacetylase sirtuin-5), also known as SIR2L5, is a 310 amino acid member of the class III sirtuins. Localized to mitochrondria and expressed throughout the body, SIRT5 is an NAD-dependent deacetylase that may link metabolic aging processes in humans. SIRT5 contains one deacetylase-sirtuin-type domain and can be deactivated by suramin, a drug that blocks the binding of various growth factors. Two isoforms of SIRT5 exist due to alternative splicing events.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human SIRT5
Antigen Species
Human
Gene Name
SIRT5
Gene ID
23408
Protein Name
NAD-dependent protein deacylase sirtuin-5, mitochondrial
Uniprot ID
Q9NXA8
Chemical Properties
Molecular WeightTheoretical: 30 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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