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Anti-SIRT3 Polyclonal Antibody is a Rabbit antibody targeting SIRT3. Anti-SIRT3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $374 | 7-10 days |
Description | Anti-SIRT3 Polyclonal Antibody is a Rabbit antibody targeting SIRT3. Anti-SIRT3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Sample: Lane 1: Panc-1 (Human) Cell Lysate at 30 μg Lane 2: 293T (Human) Cell Lysate at 30 μg Lane 3: K562 (Human) Cell Lysate at 30 μg Lane 4: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-SIRT3 (TMAB-01692) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa 2. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SIRT3 Polyclonal Antibody, Unconjugated (TMAB-01692) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SIRT3 Polyclonal Antibody, Unconjugated (TMAB-01692) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SIRT3 Polyclonal Antibody, Unconjugated (TMAB-01692) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SIRT3 Polyclonal Antibody, Unconjugated (TMAB-01692) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SIRT3 Polyclonal Antibody, Unconjugated (TMAB-01692) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SIRT3 Polyclonal Antibody, Unconjugated (TMAB-01692) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SIRT3 Polyclonal Antibody, Unconjugated (TMAB-01692) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SIRT3 Polyclonal Antibody, Unconjugated (TMAB-01692) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Mitochondrion matrix. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | SIRT3 encodes a member of the sirtuin family of class III histone deacetylases, homologs to the yeast Sir2 protein. The encoded protein is found exclusively in mitochondria, where it can eliminate reactive oxygen species, inhibit apoptosis, and prevent the formation of cancer cells. SIRT3 has far-reaching effects on nuclear gene expression, cancer, cardiovascular disease, neuroprotection, aging, and metabolic control. [provided by RefSeq, May 2019] |
Immunogen | KLH conjugated synthetic peptide: human SIRT3 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Tumor suppressors,Cardiac metabolism,Other cell division antibodies,Acetylation,Class III / Sir2 class,Obesity |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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