Anti-SIRP Alpha Polyclonal Antibody is a Rabbit antibody targeting SIRP Alpha. Anti-SIRP Alpha Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse)

1. Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-SIRP antibody (TMAB-12808), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
2. Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Cerebellum Lysates
Lane 3: Mouse Spleen Lysates
Lane 4: Mouse Placenta Lysates
Lane 5: Rat Brain Lysates
Lane 6: Rat Cerebellum Lysates
Primary: Anti-SIRP AlphA (TMAB-12808) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CD172a

Human

SIRPA

Tyrosine-protein phosphatase non-receptor type substrate 1

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.