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Anti-SIP1 Polyclonal Antibody 3
Catalog No. TMAB-12804 Copy Product Info
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Anti-SIP1 Polyclonal Antibody 3 is a Rabbit antibody targeting SIP1. Anti-SIP1 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-SIP1 Polyclonal Antibody 3
Copy Product Info🥰Excellent
Catalog No. TMAB-12804
Anti-SIP1 Polyclonal Antibody 3 is a Rabbit antibody targeting SIP1. Anti-SIP1 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-SIP1 Polyclonal Antibody 3
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-SIP1 Polyclonal Antibody 3 is a Rabbit antibody targeting SIP1. Anti-SIP1 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Cow,Horse,Sheep) |
| Verified Activity | 1. Sample: HepG2 (Human) Cell Lysate at 40 μg Primary: Anti-SIP1 (TMAB-12804) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 136 kD Observed band size: 133 kD 2. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (SIP1) Polyclonal Antibody, Unconjugated (TMAB-12804) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Concentration | 1mg/ml |
| Research Background | SMAD regulates gene expression by interacting with different classes of transcription factors including DNA-binding multi-zinc finger proteins. SIP1, for SMAD interacting protein 1, is a member of the delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SIP1 contains a SMAD-binding domain, a homeodomain and two clusters of zinc fingers on the N- and C-termini. SIP1, also known as SMADIP1, ZFHX1B and ZEB2 (zinc finger E-box-binding protein 2), can be induced by TGF∫ treatment. SIP1 plays a crucial role in normal embryonic development of neural structures and the neural crest. The human SIP1 gene maps to chromosome 2q22. Mutations in the SIP1 gene cause a form of Hirschsprung disease (HSCR). Patients with SIP1 mutations show mental retardation, delayed motor development, epilepsy, microcephaly, distinct facial features and/or congenital heart disease—all symptoms of HSCR. |
| Immunogen | KLH conjugated synthetic peptide: human SIP1 |
| Antigen Species | Human |
| Gene Name | ZEB2 |
| Gene ID | |
| Protein Name | Zinc finger E-box-binding homeobox 2 |
| Uniprot ID | |
| Function | Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin. |
| Molecular Weight | Theoretical: 136 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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