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Anti-SIGNR1 Antibody (2W828)
😃Good
Catalog No. TMAY-03317
Alias SIGNR1, OtB7, mSIGNR1, DC-SIGNR1, CD209b antigen, 1810030I22Rik
Anti-SIGNR1 Antibody (2W828) is a Rabbit antibody targeting SIGNR1. Anti-SIGNR1 Antibody (2W828) can be used in ICC/IF.
Anti-SIGNR1 Antibody (2W828)
Anti-SIGNR1 Antibody (2W828)
😃Good
Catalog No. TMAY-03317Alias SIGNR1, OtB7, mSIGNR1, DC-SIGNR1, CD209b antigen, 1810030I22Rik
Anti-SIGNR1 Antibody (2W828) is a Rabbit antibody targeting SIGNR1. Anti-SIGNR1 Antibody (2W828) can be used in ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: ICC/IF Reactivity: Mouse |
| Alias | SIGNR1, OtB7, mSIGNR1, DC-SIGNR1, CD209b antigen, 1810030I22Rik |
| Ig Type | Rabbit IgG |
| Clone | 2W828 |
| Reactivity | Mouse |
| Verified Activity | 1. Immunofluorescence staining of mouse CD209B in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD209B monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). 2. Flow cytometric analysis of Mouse CD209b expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD209b, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse SIGNR1 / CD209b (rM SIGNR1 / CD209b; TMPY-01733; NP_081248.2; Gln74-Gly325). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD209b, also known as SIGNR1, is a C-type lectin receptor. CD209b is present on most regions of mouse brain and found on microglia and dendritic cells but not on neurons or astrocytes. CD209b is implicated in the recently described SIGNR1 complement activation pathway, which operates against capsular polysaccharides in splenic marginal macrophages. CD209b in rat is homologue to SIGNR1 in mouse, both of which are found to mediate the uptake of dextran or CPS14 within the splenic marginal zone. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Mouse SIGNR1 / CD209b protein (TMPY-01733) |
| Antigen Species | Mouse |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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