Anti-SIGLEC8 Polyclonal Antibody is a Rabbit antibody targeting SIGLEC8. Anti-SIGLEC8 Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat)

1. Blank control (blue): Raji (fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-CD329 antibody (TMAB-12796), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions);
Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
2. Blank control (blue): Raji (fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-CD329 antibody (TMAB-12796), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions);
Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CD329

Human

SIGLEC8

Sialic acid-binding Ig-like lectin 8

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.