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Anti-SIAE Polyclonal Antibody
Catalog No. TMAB-12782 Copy Product Info
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Anti-SIAE Polyclonal Antibody is a Rabbit antibody targeting SIAE. Anti-SIAE Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-SIAE Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-12782
Anti-SIAE Polyclonal Antibody is a Rabbit antibody targeting SIAE. Anti-SIAE Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-SIAE Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-SIAE Polyclonal Antibody is a Rabbit antibody targeting SIAE. Anti-SIAE Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Cow,Sheep) |
| Verified Activity | 1. Sample: SW480 (Human) Cell Lysate at 40 μg Primary: Anti-SIAe (TMAB-12782) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SIAE) Polyclonal Antibody, Unconjugated (TMAB-12782) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SIAE) Polyclonal Antibody, Unconjugated (TMAB-12782) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Lysosome |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Sialic acids are acidic 9-carbon sugars typically found at the non-reducing end of sugar chains. They are frequently modified by 9-O-acetylation and this modification is removed by sialic acid acetylesterases. SIAE catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. SIAE appears to encode both lysosomal and cytosolic sialic acid acetylesterase isoforms (LSE and CSE, respectively). It is widely expressed with high expression in the testis, prostate, and colon. There are three named isoforms. |
| Immunogen | KLH conjugated synthetic peptide: human SIAE |
| Antigen Species | Human |
| Gene Name | SIAE |
| Gene ID | |
| Protein Name | Sialate O-acetylesterase |
| Uniprot ID |
| Molecular Weight | Theoretical: 56 kDa. Actual: 56 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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