Anti-SHH Polyclonal Antibody is a Rabbit antibody targeting SHH. Anti-SHH Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Cow,Horse,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (Shh) Polyclonal Antibody, Unconjugated (TMAB-01684) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
2. Sample: Embryo (Mouse) tissue at 30 μg;
Primary: Anti-Shh (TMAB-01684) at 1:300 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 5000 dilution;
Predicted band size: 19/47 kDa
Observed band size: 50 kDa
3. Sample: Stomach (Mouse) Lysate at 40 μg
Primary: Anti-Shh (TMAB-01684) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19/47 kDa
Observed band size: 49 kDa
4. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Shh) Polyclonal Antibody, Unconjugated (TMAB-01684) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Shh

Human