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Anti-Shh Antibody (2T322)

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Catalog No. TMAB-12768

Anti-Shh Antibody (2T322) is a Rabbit antibody targeting Shh. Anti-Shh Antibody (2T322) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-Shh Antibody (2T322)

Anti-Shh Antibody (2T322)

😃Good
Catalog No. TMAB-12768
Anti-Shh Antibody (2T322) is a Rabbit antibody targeting Shh. Anti-Shh Antibody (2T322) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1507-10 days7-10 days
50 μL$2627-10 days7-10 days
100 μL$4707-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Shh Antibody (2T322) is a Rabbit antibody targeting Shh. Anti-Shh Antibody (2T322) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Ig Type
IgG
Clone
2T322
Reactivity
Human
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationSonic hedgehog protein C-product: Secreted, extracellular space. Note=The C-terminal peptide diffuses from the cell. Sonic hedgehog protein N-product: Cell membrane; Lipid-anchor. Note=The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside.
Tissue SpecificityExpressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundThe C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity).Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity).Both activities occur in the reticulum endoplasmic (By similarity).
Antigen Details
Immunogen
A synthesized peptide: human Sonic hedgehog protein
Antigen Species
Human
Gene Name
SHH
Gene ID
Protein Name
Sonic hedgehog protein
Uniprot ID
Function
Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction (By similarity).
Chemical Properties
Molecular WeightTheoretical: 50 kDa. Actual: 51 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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