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Anti-SHBG Polyclonal Antibody

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Catalog No. TMAB-01683
Alias Testosterone estrogen binding globulin, Testosterone Estradiol Binding Globulin, Testosterone binding beta globulin, Testis specific androgen binding protein, TEBG, SHBG, Sex steroid binding protein, SBP, MGC138391, MGC126834, Androgen binding protein, ABP

Anti-SHBG Polyclonal Antibody is a Rabbit antibody targeting SHBG. Anti-SHBG Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-SHBG Polyclonal Antibody

Anti-SHBG Polyclonal Antibody

😃Good
Catalog No. TMAB-01683Alias Testosterone estrogen binding globulin, Testosterone Estradiol Binding Globulin, Testosterone binding beta globulin, Testis specific androgen binding protein, TEBG, SHBG, Sex steroid binding protein, SBP, MGC138391, MGC126834, Androgen binding protein, ABP
Anti-SHBG Polyclonal Antibody is a Rabbit antibody targeting SHBG. Anti-SHBG Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-SHBG Polyclonal Antibody is a Rabbit antibody targeting SHBG. Anti-SHBG Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasTestosterone estrogen binding globulin, Testosterone Estradiol Binding Globulin, Testosterone binding beta globulin, Testis specific androgen binding protein, TEBG, SHBG, Sex steroid binding protein, SBP, MGC138391, MGC126834, Androgen binding protein, ABP
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Cow,Rabbit)
Verified Activity
1. Sample:
Lane1: Testis (Mouse) Lysate at 30 μg
Lane2: Liver (Mouse) Lysate at 30 μg
Primary: Anti-ABP/SHBg (TMAB-01683) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000 dilution;
Predicted band size: 40 kDa
Observed band size: 44 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (TMAB-01683) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (TMAB-01683) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (TMAB-01683) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
5. Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human U87MG cell lysates
Primary: Anti-SHBG (TMAB-01683) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 50 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted . Note=In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundSex hormone binding globulin (SHBG) is a glycoprotein synthesized by the liver. Circulating androgen and estrogen concentrations influence SHBG synthesis. Elevated testosterone, for example, causes SHBG synthesis to decrease, whereas high estrogen stimulates SHBG production. The regulation of SHBG synthesis, combined with SHBG's higher affinity for testosterone, impacts bioavailable testosterone levels. SHBG binds up to 98 percent of the steroid hormones in the blood including 5a-dihydrotestosterone (DHT), testosterone and androstenediol with particularly high affinity, and estradiol and estrone with slightly lower affinity.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human SHBG
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cancer,Hormones,Lipid metabolism
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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