Anti-SH3D19 Polyclonal Antibody is a Rabbit antibody targeting SH3D19. Anti-SH3D19 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Cow,Horse,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SH3D19) Polyclonal Antibody, Unconjugated (TMAB-12752) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SH3D19) Polyclonal Antibody, Unconjugated (TMAB-12752) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Sample: Heart (mouse) Cell Lysate at 40 μg
Primary: Anti-SH3D19 (TMAB-12752) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 86 kD
Observed band size:86 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human SH3D19

Human

SH3D19

SH3 domain-containing protein 19

May play a role in regulating A disintegrin and metalloproteases (ADAMs) in the signaling of EGFR-ligand shedding. May be involved in suppression of Ras-induced cellular transformation and Ras-mediated activation of ELK1.