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Anti-SGSH Polyclonal Antibody
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Catalog No. TMAB-12740
Anti-SGSH Polyclonal Antibody is a Rabbit antibody targeting SGSH. Anti-SGSH Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-SGSH Polyclonal Antibody
Anti-SGSH Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-12740
Anti-SGSH Polyclonal Antibody is a Rabbit antibody targeting SGSH. Anti-SGSH Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-SGSH Polyclonal Antibody is a Rabbit antibody targeting SGSH. Anti-SGSH Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog) |
| Verified Activity | 1. Sample: Lane 1: Mouse NIH/3T3 cell lysates Lane 2: Human MCF-7 cell lysates Lane 3: Human 293T cell lysates Lane 4: Human HUVEC cell lysates Lane 5: Human HeLa cell lysates Primary: Anti-SGSH (TMAB-12740) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 60 kDa 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-SGSH Polyclonal Antibody, Unconjugated (TMAB-12740) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Lysosome. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Sulfatases are enzymes that hydrolyse a diverse range of sulfate esters. Deficiency of lysosomal sulfatases leads to human diseases characterized by the accumulation of either GAGs (glycosaminoglycans) or sulfolipids. Sulfamidase, also known as HSS, SFMD, MPS3A or SGSH, is a 502 amino acid lysosome that belongs to the sulfatase family. It has been suggested that sulfamidase may be involved in the lysosomal degradation of heparan sulfate. Defects in the gene encoding sulfamidase are the cause of Sanfilippo syndrome A, an autosomal recessive lysosomal storage disease caused by impaired degradation of heparan sulfate. Sanfilippo syndrome A is characterized by severe central nervous system degeneration but relatively mild somatic manifestations. |
| Immunogen | KLH conjugated synthetic peptide: human Sulphamidase |
| Antigen Species | Human |
| Gene Name | SGSH |
| Gene ID | |
| Protein Name | N-sulphoglucosamine sulphohydrolase |
| Uniprot ID |
| Molecular Weight | Theoretical: 55 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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