Anti-SFTPC Polyclonal Antibody is a Rabbit antibody targeting SFTPC. Anti-SFTPC Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Cow,Rabbit,Sheep)

1. Cell: NCI-H460
Concentration:1:100
Host/Isotype: Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: TMAB-01682) on NCI-H460 (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit Igg (H+L) secondary antibody.
2. Blank control (black line): A549. Primary Antibody (green line): Rabbit Anti-SFTPC antibody (TMAB-01682)-PE
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Negative control (white blue line): PBS
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human SFTPC

Human