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Anti-SETD7 Antibody (8B368)

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Catalog No. TMAY-01753
Alias SET9, SET7/9, SET7, SET domain containing (lysine methyltransferase) 7, KMT7

Anti-SETD7 Antibody (8B368) is a Mouse antibody targeting SETD7. Anti-SETD7 Antibody (8B368) can be used in WB.

Anti-SETD7 Antibody (8B368)

Anti-SETD7 Antibody (8B368)

😃Good
Catalog No. TMAY-01753Alias SET9, SET7/9, SET7, SET domain containing (lysine methyltransferase) 7, KMT7
Anti-SETD7 Antibody (8B368) is a Mouse antibody targeting SETD7. Anti-SETD7 Antibody (8B368) can be used in WB.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-SETD7 Antibody (8B368) is a Mouse antibody targeting SETD7. Anti-SETD7 Antibody (8B368) can be used in WB.
AliasSET9, SET7/9, SET7, SET domain containing (lysine methyltransferase) 7, KMT7
Ig Type
Monoclonal Mouse IgG1
Clone
8B368
Reactivity
Human
Verified Activity
Anti-SETD7 mouse monoclonal antibody at 1:500 dilution.

-Lane A: Hela Whole Cell Lysate.

-Lane B: MCF7 Whole Cell Lysate.

-Lane C: NIH3T3 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:41 kDa.

-Observed band size:53 kDa(We are unsure as to the identity of these extra bands.)
Application
WB
Recommended Dose
WB: 1:500-1:1000
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SETD7 / SET7 / 9 (rh SETD7 / SET7 / 9; TMPY-01229; NP_085151.1; Asp2-Lys366). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundHistone-lysine N-methyltransferase SETD7, also known as SET domain containing (lysine methyltransferase) 7, SET7/9, Histone H3-K4 methyltransferase SETD7, H3-K4-HMTase SETD7, and SETD7, is a member of the histone-lysine methyltransferase family and SET7 subfamily. SETD7 is widely expressed and expressed in pancreatic islets. SETD7 contains three MORN repeats and one SET domain. SETD7 plays a central role in the transcriptional activation of genes such as collagenase or insulin. As a protein lysine methyltransferase (PKMT), SETD7 also has methyltransferase activity toward non-histone proteins such as p53/TP53, TAF1, and possibly TAF7 by recognizing and binding in substrate proteins. The mono-methyltransferase activity of SETD7 is achieved by disrupting the formation at near-attack conformations for the dimethylation reaction. SETD7 is also a novel coactivator of NF-kappaB and plays a role in inflammation and diabetes.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human SETD7 / SET7 / 9 protein (TMPY-01229)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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