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Anti-SERPINB3 Antibody (2E52)
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Catalog No. TMAB-12686
Anti-SERPINB3 Antibody (2E52) is a Rabbit antibody targeting SERPINB3. Anti-SERPINB3 Antibody (2E52) can be used in WB, IHC-P, IHC-Fr, IF.
Anti-SERPINB3 Antibody (2E52)
Anti-SERPINB3 Antibody (2E52)
Copy Product Info😃Good
Catalog No. TMAB-12686
Anti-SERPINB3 Antibody (2E52) is a Rabbit antibody targeting SERPINB3. Anti-SERPINB3 Antibody (2E52) can be used in WB, IHC-P, IHC-Fr, IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-SERPINB3 Antibody (2E52) is a Rabbit antibody targeting SERPINB3. Anti-SERPINB3 Antibody (2E52) can be used in WB, IHC-P, IHC-Fr, IF. |
| Ig Type | IgG |
| Clone | 2E52 |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SERPINB3 Monoclonal Antibody, Unconjugated (TMAB-12686) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SERPINB3 Monoclonal Antibody, Unconjugated (TMAB-12686) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SERPINB3 Monoclonal Antibody, Unconjugated (TMAB-12686) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. 25 μg total protein per lane of various lysates (see on figure) probed with SERPINB3 monoclonal antibody, unconjugated (TMAB-12686) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC-P: 1:200-800; IHC-Fr: 1:200-800; IF: 1:200-800 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Seems to also be secreted in plasma by cancerous cells but at a low level. |
| Tissue Specificity | Squamous cells. Expressed in some hepatocellular carcinoma (at protein level). |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Metastasis of a primary tumor to a distant site is determined through signaling cascades that break down interactions between the cell and extracellular matrix proteins. Among the proteins mediating metastasis are serine prote-ases, such as neutrophil elastase.SCCA is transcribed by two nearly identical genes (SCCA1 and SCCA2), and is mainly produced as SCCA1. The human SCCA1 gene encodes a 390 amino acid protein that was originally isolated from a metastatic cervical squamous cell carcinoma. |
| Immunogen | Recombinant Protein: human SERPINB3 protein |
| Antigen Species | Human |
| Gene Name | SERPINB3 |
| Gene ID | |
| Protein Name | Serpin B3 |
| Uniprot ID | |
| Function | May act as a protease inhibitor to modulate the host immune response against tumor cells. |
| Molecular Weight | Theoretical: 45 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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