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Anti-SERPINB1 Antibody (7K553)
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Catalog No. TMAY-02386
Anti-SERPINB1 Antibody (7K553) is a Mouse antibody targeting SERPINB1. Anti-SERPINB1 Antibody (7K553) can be used in WB,IP.
Anti-SERPINB1 Antibody (7K553)
Anti-SERPINB1 Antibody (7K553)
😃Good
Catalog No. TMAY-02386
Anti-SERPINB1 Antibody (7K553) is a Mouse antibody targeting SERPINB1. Anti-SERPINB1 Antibody (7K553) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, IP Reactivity: Human |
| Ig Type | Mouse IgG1 |
| Clone | 7K553 |
| Reactivity | Human |
| Verified Activity | 1. Anti-SERPINB1 mouse monoclonal antibody at 1:500 dilution. -Lane A: NCI-H460 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:43 kDa. -Observed band size:43 kDa. 2. SERPINB1 was immunoprecipitated using: -Lane A:0.5 mg 293T Whole Cell Lysate -0.5 µL anti-SERPINB1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-SERPINB1 mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 43 kDa. -Observed band size: 43 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:1000; IP: 1-2 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SerpinB1 / ELANH2 (rh SerpinB1 / ELANH2; TMPY-02128; P30740; Met1-Pro379). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | SerpinB1 is an endogenous inhibitor of serine proteases recognized for its anti-inflammatory and host-protective properties. Serum SerpinB1 levels are elevated in patients with type 2 diabetes compared with that in healthy subjects and are negatively correlated with serum LDL-C. serpinB1 as an endogenous protein that can potentially be harnessed to enhance functional beta cell mass in patients with diabetes. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human SerpinB1 / ELANH2 protein (TMPY-02128) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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