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Anti-SERPINB1 Antibody (7K553)

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Catalog No. TMAY-02386

Anti-SERPINB1 Antibody (7K553) is a Mouse antibody targeting SERPINB1. Anti-SERPINB1 Antibody (7K553) can be used in WB,IP.

Anti-SERPINB1 Antibody (7K553)

Anti-SERPINB1 Antibody (7K553)

😃Good
Catalog No. TMAY-02386
Anti-SERPINB1 Antibody (7K553) is a Mouse antibody targeting SERPINB1. Anti-SERPINB1 Antibody (7K553) can be used in WB,IP.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-SERPINB1 Antibody (7K553) is a Mouse antibody targeting SERPINB1. Anti-SERPINB1 Antibody (7K553) can be used in WB,IP.
Ig Type
Monoclonal Mouse IgG1
Clone
7K553
Reactivity
Human
Verified Activity
1. Anti-SERPINB1 mouse monoclonal antibody at 1:500 dilution.

-Lane A: NCI-H460 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:43 kDa.

-Observed band size:43 kDa.

2. SERPINB1 was immunoprecipitated using:

-Lane A:0.5 mg 293T Whole Cell Lysate

-0.5 µL anti-SERPINB1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-SERPINB1 mouse monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 43 kDa.

-Observed band size: 43 kDa
Application
WB,IP
Recommended Dose
WB: 1:500-1:1000; IP: 1-2 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SerpinB1 / ELANH2 (rh SerpinB1 / ELANH2; TMPY-02128; P30740; Met1-Pro379). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundSerpinB1 is an endogenous inhibitor of serine proteases recognized for its anti-inflammatory and host-protective properties. Serum SerpinB1 levels are elevated in patients with type 2 diabetes compared with that in healthy subjects and are negatively correlated with serum LDL-C. serpinB1 as an endogenous protein that can potentially be harnessed to enhance functional beta cell mass in patients with diabetes.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human SerpinB1 / ELANH2 protein (TMPY-02128)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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