Anti-SERCA1 Antibody (3G166) is a Rabbit antibody targeting SERCA1. Anti-SERCA1 Antibody (3G166) can be used in WB,IHC-P,IHC-Fr,IP,IF.

IgG

3G166

Human

1. 25 μg total protein per lane of various lysates (see on figure) probed with SERCA1 monoclonal antibody, unconjugated (TMAB-12666) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SERCA1 Monoclonal Antibody, Unconjugated (TMAB-12666) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SERCA1 Monoclonal Antibody, Unconjugated (TMAB-12666) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IP: 1:20-50; IF: 1:100-500

Monoclonal

A synthesized peptide: human SERCA1

Human

ATP2A1

Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.