Anti-SEPT2 Antibody (3M4) is a Mouse antibody targeting SEPT2. Anti-SEPT2 Antibody (3M4) can be used in WB,IHC-P,IHC-Fr,IF.

IgG1, Kappa

3M4

Human (predicted:Mouse,Rat)

1. Tissue: Human liver cancer
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for TMAB-12661
2. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition:4°C overnight
Lysate: 293, Jurkat, 3T3
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 42 kDa
Observed MW: 50 kDa

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

SEPTIN2

Septin-2

Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.