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Anti-Semaphorin 3F Polyclonal Antibody 2
Catalog No. TMAB-12648 Copy Product Info
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Anti-Semaphorin 3F Polyclonal Antibody 2 is a Rabbit antibody targeting Semaphorin 3F. Anti-Semaphorin 3F Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Semaphorin 3F Polyclonal Antibody 2
Copy Product Info🥰Excellent
Catalog No. TMAB-12648
Anti-Semaphorin 3F Polyclonal Antibody 2 is a Rabbit antibody targeting Semaphorin 3F. Anti-Semaphorin 3F Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Semaphorin 3F Polyclonal Antibody 2
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-Semaphorin 3F Polyclonal Antibody 2 is a Rabbit antibody targeting Semaphorin 3F. Anti-Semaphorin 3F Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Sample: Lane 1: Mouse Brain Lysates Lane 2: Rat Brain Lysates Primary: Anti-Semaphorin 3F (TMAB-12648) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kDa Observed band size: 100 kDa 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Semaphorin 3F) Polyclonal Antibody, Unconjugated (TMAB-12648) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Tissue Specificity | Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues(Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family. |
| Immunogen | KLH conjugated synthetic peptide: human Semaphorin 3F |
| Antigen Species | Human |
| Gene Name | SEMA3F |
| Gene ID | |
| Protein Name | Semaphorin-3F |
| Uniprot ID | |
| Function | May play a role in cell motility and cell adhesion. |
| Molecular Weight | Theoretical: 88 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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