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Anti-SEMA4A Polyclonal Antibody

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Catalog No. TMAB-01674

Anti-SEMA4A Polyclonal Antibody is a Rabbit antibody targeting SEMA4A. Anti-SEMA4A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-SEMA4A Polyclonal Antibody

Anti-SEMA4A Polyclonal Antibody

😃Good
Catalog No. TMAB-01674
Anti-SEMA4A Polyclonal Antibody is a Rabbit antibody targeting SEMA4A. Anti-SEMA4A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-SEMA4A Polyclonal Antibody is a Rabbit antibody targeting SEMA4A. Anti-SEMA4A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SEMA4A) Polyclonal Antibody, Unconjugated (TMAB-01674) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Kidney (Mouse) Lysate at 40 μg
Primary: Anti-SEMA4A (TMAB-01674) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 83 kDa
3. Sample:
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-SEMA4A (TMAB-01674) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 83 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Single-pass type I membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundSEMA4A belongs to the semaphorin family of soluble and transmembrane proteins. These proteins play a role in guidance of axonal migration during neuronal development and in immune responses.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human SEMA4A
Antigen Species
Human
Gene ID
Biology Area
Inhibitors,Axonal Guidance Proteins,Visual system
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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