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Anti-SEMA4A Polyclonal Antibody
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Catalog No. TMAB-01674
Alias SEMB, semaphorin 4A, SEMAB, RP35, CORD10
Anti-SEMA4A Polyclonal Antibody is a Rabbit antibody targeting SEMA4A. Anti-SEMA4A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-SEMA4A Polyclonal Antibody
Anti-SEMA4A Polyclonal Antibody
😃Good
Catalog No. TMAB-01674Alias SEMB, semaphorin 4A, SEMAB, RP35, CORD10
Anti-SEMA4A Polyclonal Antibody is a Rabbit antibody targeting SEMA4A. Anti-SEMA4A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-SEMA4A Polyclonal Antibody is a Rabbit antibody targeting SEMA4A. Anti-SEMA4A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | SEMB, semaphorin 4A, SEMAB, RP35, CORD10 |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SEMA4A) Polyclonal Antibody, Unconjugated (TMAB-01674) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Kidney (Mouse) Lysate at 40 μg Primary: Anti-SEMA4A (TMAB-01674) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kDa Observed band size: 83 kDa 3. Sample: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-SEMA4A (TMAB-01674) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kDa Observed band size: 83 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | SEMA4A belongs to the semaphorin family of soluble and transmembrane proteins. These proteins play a role in guidance of axonal migration during neuronal development and in immune responses. |
| Immunogen | KLH conjugated synthetic peptide: human SEMA4A |
| Antigen Species | Human |
| Gene ID | |
| Biology Area | Inhibitors,Axonal Guidance Proteins,Visual system |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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