Anti-SEMA4A Polyclonal Antibody is a Rabbit antibody targeting SEMA4A. Anti-SEMA4A Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SEMA4A) Polyclonal Antibody, Unconjugated (TMAB-01674) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Kidney (Mouse) Lysate at 40 μg
Primary: Anti-SEMA4A (TMAB-01674) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 83 kDa
3. Sample:
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-SEMA4A (TMAB-01674) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 83 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human SEMA4A

Human

SEMA4A

Semaphorin-4A

Inhibitors,Axonal Guidance Proteins,Visual system

Cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. Plays a role in priming antigen-specific T cells, promotes differentiation of Th1 T helper cells, and thereby contributes to adaptive immunity. Promotes phosphorylation of TIMD2. Inhibits angiogenesis. Promotes axon growth cone collapse. Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons (By similarity).