Anti-Securin Polyclonal Antibody is a Rabbit antibody targeting Securin. Anti-Securin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Pig,Horse)

1. Protein: testis (mouse) lysate at 30 μg;
Primary: rabbit Anti-Securin (TMAB-12632) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000;
Predicted band size: 28 kD
Observed band size: 28 kD
2. Protein: human colon carcinoma lysates at 30 μg;
Primary: Rabbit Anti-Securin (TMAB-12632) at 1: 200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 5000;
ECL excitated the fluorescence;
Predicted band size: 23 kD
Observed band size: 23 kD
3. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Securin) Polyclonal Antibody, Unconjugated (TMAB-12632) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Securin) Polyclonal Antibody, Unconjugated (TMAB-12632) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Securin) Polyclonal Antibody, Unconjugated (TMAB-12632) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PTTG1

Human

PTTG1

Securin

Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.