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Anti-SDHB Antibody (3I144)
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Catalog No. TMAC-03698
Alias Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, SDH1, SDH, mitochondrial, Iron-sulfur subunit of complex II, Ip, EC 1.3.5.1
Anti-SDHB Antibody (3I144) is a Rabbit antibody targeting SDHB. Anti-SDHB Antibody (3I144) can be used in WB,IHC,FCM,IP.
Anti-SDHB Antibody (3I144)
Anti-SDHB Antibody (3I144)
😃Good
Catalog No. TMAC-03698Alias Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, SDH1, SDH, mitochondrial, Iron-sulfur subunit of complex II, Ip, EC 1.3.5.1
Anti-SDHB Antibody (3I144) is a Rabbit antibody targeting SDHB. Anti-SDHB Antibody (3I144) can be used in WB,IHC,FCM,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-SDHB Antibody (3I144) is a Rabbit antibody targeting SDHB. Anti-SDHB Antibody (3I144) can be used in WB,IHC,FCM,IP. |
| Synonyms | Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, SDH1, SDH, mitochondrial, Iron-sulfur subunit of complex II, Ip, EC 1.3.5.1 |
| Ig Type | IgG |
| Clone | 3I144 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of SDHB on different lysates using anti-SDHB antibody at 1/500 dilution. Positive control: Lane 1: Human liver, Lane 2: Rat spleen, Lane 3: Rat liver, Lane 4: Mouse spleen, Lane 5: HepG2. 2. Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-SDHB antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHB antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SDHB antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHB antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-SDHB antibody. Counter stained with hematoxylin. 7. Flow cytometric analysis of Jurkat cells with SDHB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations associate with juvenile encephalopathy as well as Leigh syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 32 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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