Anti-SCARB1/Scavenger Receptor BI Antibody (8A412) is a Rabbit antibody targeting SCARB1/Scavenger Receptor BI. Anti-SCARB1/Scavenger Receptor BI Antibody (8A412) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

8A412

Human,Mouse,Rat

1. Cell line: HepG2
Fixation: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-12560
2. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Adrenal gland tissue lysates
Lane 3: Rat Liver tissue lysates
Lane 4: Rat Adrenal gland tissue lysates
Lane 5: Human HepG2 cell lysates
Lane 6: Human HeLa cell lysates
Primary: Anti-SCARB1/Scavenger Receptor BI (TMAB-12560) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 75 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (SCARB1/Scavenger Receptor BI) Monoclonal Antibody, Unconjugated (TMAB-12560) at 1: 2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (SCARB1/Scavenger Receptor BI) Monoclonal Antibody, Unconjugated (TMAB-12560) at 1: 2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (SCARB1/Scavenger Receptor BI) Monoclonal Antibody, Unconjugated (TMAB-12560) at 1: 2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (SCARB1/Scavenger Receptor BI) Monoclonal Antibody, Unconjugated (TMAB-12560) at 1: 2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (SCARB1/Scavenger Receptor BI) Monoclonal Antibody, Unconjugated (TMAB-12560) at 1: 2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:200-500; ICC/IF: 1:50-200; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human SCARB1/Scavenger Receptor BI

Human

SCARB1

Scavenger receptor class B member 1

Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester.