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Anti-SARS-CoV-2 N Polyclonal Antibody
😃Good
Catalog No. TMAB-01663
Alias Surface glycoprotein, SPIKE_SARS2, Spike protein S1, Spike protein RBD, Spike glycoprotein, SARS-CoV-2 spike protein, 2019-nCOV Spike protein
Anti-SARS-CoV-2 N Polyclonal Antibody is a Rabbit antibody targeting SARS-CoV-2 N. Anti-SARS-CoV-2 N Polyclonal Antibody can be used in WB.
Anti-SARS-CoV-2 N Polyclonal Antibody
Anti-SARS-CoV-2 N Polyclonal Antibody
😃Good
Catalog No. TMAB-01663Alias Surface glycoprotein, SPIKE_SARS2, Spike protein S1, Spike protein RBD, Spike glycoprotein, SARS-CoV-2 spike protein, 2019-nCOV Spike protein
Anti-SARS-CoV-2 N Polyclonal Antibody is a Rabbit antibody targeting SARS-CoV-2 N. Anti-SARS-CoV-2 N Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μg | $487 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-SARS-CoV-2 N Polyclonal Antibody is a Rabbit antibody targeting SARS-CoV-2 N. Anti-SARS-CoV-2 N Polyclonal Antibody can be used in WB. |
| Synonyms | Surface glycoprotein, SPIKE_SARS2, Spike protein S1, Spike protein RBD, Spike glycoprotein, SARS-CoV-2 spike protein, 2019-nCOV Spike protein |
| Ig Type | IgG |
| Reactivity | (predicted:SARS-CoV-2) |
| Verified Activity | 1. Sample: Lane 1: SARS-CoV-2 N protein at 50ng Lane 2: SARS-CoV-2 N protein at 25ng Lane 3: SARS-CoV-2 N protein at 15ng Lane 4: SARS-CoV-2 N protein at 10ng Lane 5: SARS-CoV-2 N protein at 5ng Lane 6: SARS-CoV-2 N protein at 2.5ng Primary: Anti-SARS-CoV-2 N protein (TMAB-01663) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa 2. Sample: Lane 1: SARS-CoV N protein at 50ng Lane 2: SARS-CoV N protein at 25ng Lane 3: SARS-CoV N protein at 15ng Lane 4: SARS-CoV N protein at 10ng Lane 5: SARS-CoV N protein at 5ng Lane 6: SARS-CoV N protein at 2.5ng Lane 7: SARS-CoV-2 N protein at 50ng Lane 8: SARS-CoV-2 N protein at 25ng Lane 9: SARS-CoV-2 N protein at 15ng Lane 10: SARS-CoV-2 N protein at 10ng Lane 11: SARS-CoV-2 N protein at 5ng Lane 12: SARS-CoV-2 N protein at 2.5ng Primary: Anti-SARS-CoV-2 N protein (TMAB-01663) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Virion membrane ; Single-pass type I membrane protein ; Host endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein ; Host cell membrane ; Single-pass type I membrane protein ;Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion. |
| Tissue Specificity | The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes.Specific enzymatic cleavages in vivo yield mature proteins. The precurssor is processed into S1 and S2 by host cell furin or another cellular protease t |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | ≥1 mg/mL |
| Research Background | The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct. |
| Immunogen | Recombinant Protein: SARS-CoV-2 Nucleocapsid Protein |
| Gene Name | S |
| Protein Name | Spike glycoprotein |
| Biology Area | SARS Coronavirus |
| Function | attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. |
| Molecular Weight | Theoretical: 46 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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