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Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (5C944)

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Catalog No. TMAB-01657

Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (5C944) is a Mouse antibody targeting SARS-CoV-2 (2019-nCoV) Nucleocapsid. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (5C944) can be used in WB.

Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (5C944)

Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (5C944)

😃Good
Catalog No. TMAB-01657
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (5C944) is a Mouse antibody targeting SARS-CoV-2 (2019-nCoV) Nucleocapsid. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (5C944) can be used in WB.
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100 μg$489 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Mouse Monoclonal

Application: WB

Reactivity: (predicted:SARS-CoV-2)
Ig Type
IgG1
Clone
5C944
Reactivity
(predicted:SARS-CoV-2)
Verified Activity
1. Sample:
Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag)
Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag)
Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag)
Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag)
Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag)
Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag)
Primary: Anti-SARS-CoV-2 (2019-nCoV) nucleocapsid (TMAB-01657) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 52 kDa
2. Sample:
Lane 1: SARS-CoV-2 N protein at 50ng
Lane 2: SARS-CoV-2 N protein at 25ng
Lane 3: SARS-CoV-2 N protein at 5ng
Lane 4: SARS-CoV-2 N protein at 2ng
Lane 5: SARS-CoV-2 N protein at 1ng
Lane 6: SARS-CoV-2 N protein at 0.5ng
Primary: Anti-SARS-CoV-2 N protein at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
3. Sample:
Lane 1: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 100ng
Lane 2: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 50ng
Lane 3: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 25ng
Lane 4: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 10ng
Lane 5: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 5ng
Lane 6: Recombinant SARS-CoV-2 N Protein (WT) at 10ng
Primary: Mouse Anti-SARS-CoV-2 N protein (TMAB-01657) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size:45.3 kDa
Observed band size:50 kD
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationVirion membrane ; Single-pass type I membrane protein ; Host endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein ; Host cell membrane ; Single-pass type I membrane protein ;Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M PBS (pH7.4) with 0.02% Proclin300.
Research BackgroundThe SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.
Antigen Details
Immunogen
Recombinant Protein: SARS-CoV-2 Nucleocapsid Protein
Biology Area
SARS Coronavirus
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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