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Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747)
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Catalog No. TMAB-01655
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747) is a Mouse antibody targeting SARS-CoV-2 (2019-nCoV) Nucleocapsid. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747) can be used in WB.
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747)
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747)
😃Good
Catalog No. TMAB-01655
Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747) is a Mouse antibody targeting SARS-CoV-2 (2019-nCoV) Nucleocapsid. Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody (1Z747) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μg | $489 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB Reactivity: SARS-CoV-2 |
| Ig Type | IgG2b |
| Clone | 1Z747 |
| Reactivity | SARS-CoV-2 |
| Verified Activity | 1. Sample: Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag) Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag) Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag) Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag) Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag) Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag) Primary: Anti-SARS-CoV-2 (2019-nCoV) nucleocapsid (TMAB-01655) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 48 kDa 2. Sample: Lane 1: SARS-CoV-2 N protein at 50ng Lane 2: SARS-CoV-2 N protein at 25ng Lane 3: SARS-CoV-2 N protein at 5ng Lane 4: SARS-CoV-2 N protein at 2ng Lane 5: SARS-CoV-2 N protein at 1ng Lane 6: SARS-CoV-2 N protein at 0.5ng Primary: Anti-SARS-CoV-2 N protein at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa 3. Sample: Lane 1: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 100ng Lane 2: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 50ng Lane 3: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 25ng Lane 4: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 10ng Lane 5: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L,E31del,R32del, S33del,R203K,G204R) at 5ng Lane 6: Recombinant SARS-CoV-2 N Protein (WT) at 10ng Primary: Mouse Anti-SARS-CoV-2 N protein (TMAB-01655) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size:45.3 kDa Observed band size:50 kD |
| Application | WB |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Virion membrane ; Single-pass type I membrane protein ; Host endoplasmic reticulum-Golgi intermediate compartment membrane ; Single-pass type I membrane protein ; Host cell membrane ; Single-pass type I membrane protein ;Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| Research Background | The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct. |
| Immunogen | Recombinant Protein: SARS-CoV-2 Nucleocapsid Protein |
| Biology Area | SARS Coronavirus |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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